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# Pileup for PacBio/ONT requires relaxing BAQ (Base Alignment Quality) calculation # because the alignment errors differ from short reads. bcftools mpileup -f reference.fa -a AD,DP longread.bam | bcftools call -mv -o variants.vcf
Additionally, Samtool will introduce automatic operator substitution for unsupported ops using MLIR dialects. samtool supported models
A SAMtools-supported model is rarely used in isolation. It serves as the foundation for three high-level models: # Pileup for PacBio/ONT requires relaxing BAQ (Base
# Sort using a memory buffer appropriate for the model's output samtools sort -@ 8 -m 4G -o sorted.bam output.bam samtool supported models